Ultrahigh-Throughput Multiplexing and Sequencing of >500-Base-Pair Amplicon Regions on the Illumina HiSeq 2500 Platform

ABSTRACT Amplification, sequencing, and analysis of the 16S rRNA gene affords characterization of microbial community composition. As this tool has become more popular and amplicon-sequencing applications have grown in the total number of samples, growth in sample multiplexing is becoming necessary...

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Autors principals:	Johanna B. Holm, Michael S. Humphrys, Courtney K. Robinson, Matthew L. Settles, Sandra Ott, Li Fu, Hongqiu Yang, Pawel Gajer, Xin He, Elias McComb, Patti E. Gravitt, Khalil G. Ghanem, Rebecca M. Brotman, Jacques Ravel
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology 2019-02-01
Col·lecció:	mSystems
Matèries:	16S RNA amplicon sequencing PCR ultrahigh throughput
Accés en línia:	https://journals.asm.org/doi/10.1128/msystems.00029-19
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