Generation of renewable mouse intestinal epithelial cell monolayers and organoids for functional analyses
Abstract Background Conditional reprogramming has enabled the development of long-lived, normal epithelial cell lines from mice and humans by in vitro culture with ROCK inhibitor on a feeder layer. We applied this technology to mouse small intestine to create 2D mouse intestinal epithelial monolayer...
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| Principais autores: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BMC
2018-08-01
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| coleção: | BMC Cell Biology |
| Assuntos: | |
| Acesso em linha: | http://link.springer.com/article/10.1186/s12860-018-0165-0 |
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