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A comparative study of extraction free detection of HBV DNA using sodium dodecyl sulfate, N-lauroylsarcosine sodium salt, and sodium dodecyl benzene sulfonate

Abstract This study aimed to develop an extraction-free method for quantitative and qualitative detection of HBV DNA compared to traditional nucleic acid extraction. Paired serum and dried blood spot (DBS) samples from 67 HBsAg-positive and 67 HBsAg-negative individuals were included. Two samples wi...

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Principais autores: Ko Ko, Lyubov Mikhailovna Lokteva, Golda Ataa Akuffo, Zayar Phyo, Chanroth Chhoung, E. Bunthen, Serge Ouoba, Aya Sugiyama, Tomoyuki Akita, Kim Rattana, Ork Vichit, Kazuaki Takahashi, Junko Tanaka
Format: Artigo
Sprog:Inglês
Udgivet: Nature Portfolio 2024-10-01
Serier:Scientific Reports
Fag:
HBV DNA
qPCR
Surfactant
Sodium dodecyl sulfate
N-Lauroylsarcosine sodium salt
Sodium dodecyl benzene sulfonate
Online adgang:https://doi.org/10.1038/s41598-024-75944-7
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