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High Efficiency RNA Extraction From Sperm Cells Using Guanidinium Thiocyanate Supplemented With Tris(2-Carboxyethyl)Phosphine

The extraction of high-quality ribonucleic acid (RNA) from tissues and cells is a key step in many biological assays. Guanidinium thiocyanate-phenol-chloroform (AGPC) is a widely used and efficient method to obtain pure RNA from most tissues and cells. However, it is not efficient with some cells li...

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Principais autores: Martin Roszkowski, Isabelle M. Mansuy
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2021-04-01
Colecção:Frontiers in Cell and Developmental Biology
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Acesso em linha:https://www.frontiersin.org/articles/10.3389/fcell.2021.648274/full
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