Protocol to correlate electron microscopy with electrophysiology in single-cell autaptic microcultures

Summary: Single-cell microcultures (SCMs) form a monosynaptic circuit that allows stimulation and recording of postsynaptic responses using a single electrode. Here, we present a protocol to establish autaptic cultures from rat superior cervical ganglion neurons. We describe the steps for preparing...

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Autores principales:	Pablo Martínez San Segundo, Anna Priscil·la Pérez González, Cecilia D. Velasco, Artur Llobet
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	Elsevier 2024-06-01
Colección:	STAR Protocols
Materias:	Cell Biology Cell culture Single Cell Neuroscience
Acceso en línea:	http://www.sciencedirect.com/science/article/pii/S2666166724001680
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