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Quantification of T Cell Antigen-specific Memory Responses in Rhesus Macaques, Using Cytokine Flow Cytometry (CFC, also Known as ICS and ICCS): from Assay Set-up to Data Acquisition

What was initially termed ‘CFC’ (Cytokine Flow Cytometry’) is now more commonly known as ‘ICS’ (Intra Cellular Staining), or less commonly as ‘ICCS’ (Intra Cellular Cytokine Staining). The key innovations were use of an effective permeant (allowing intracellular staining), and a reagent to disrupt s...

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Autors principals: Andrew Sylwester, Scott Hansen, Louis Picker
Format: Artigo
Idioma:Inglês
Publicat: Bio-protocol LLC 2014-04-01
Col·lecció:Bio-Protocol
Accés en línia:https://bio-protocol.org/en/bpdetail?id=1110&type=0
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