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Construction of Marker-Free Transgenic Strains of Chlamydomonas reinhardtii Using a Cre/loxP-Mediated Recombinase System.

The Escherichia coli bacteriophage P1 encodes a site-specific recombinase called Cre and two 34-bp target sites of Cre recombinase called loxP. The Cre/loxP system has been used to achieve targeted insertion and precise deletion in many animal and plant genomes. The Cre/loxP system has particularly...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Hauptverfasser: Yuki Kasai, Shigeaki Harayama
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science (PLoS) 2016-01-01
Schriftenreihe:PLoS ONE
Online-Zugang:http://europepmc.org/articles/PMC5001723?pdf=render
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