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Isolation of native plasma membrane H+‐ATPase (Pma1p) in both the active and basal activation states

The yeast plasma membrane H+‐ATPase Pma1p is a P‐type ATPase that energizes the yeast plasma membrane. Pma1p exists in two activation states: an autoinhibited basal state and an activated state. Here we show that functional and stable Pma1p can be purified in native form and reconstituted in artific...

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Autores principales: Jesper Torbøl Pedersen, Tamara Kanashova, Gunnar Dittmar, Michael Palmgren
Formato: Artigo
Lenguaje:Inglês
Publicado: Wiley 2018-05-01
Colección:FEBS Open Bio
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Acceso en línea:https://doi.org/10.1002/2211-5463.12413
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