CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome

CRISPR/Cas9 gene editing has been used to generate mutations in several mouse genes. Here, the authors show that targeting events using single guide RNAs cause large deletions at 17 sites in the mouse genome, suggesting that careful genotyping is needed and sequential targeting may avoid such deleti...

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Bibliografische gegevens

Hoofdauteurs:	Ha Youn Shin, Chaochen Wang, Hye Kyung Lee, Kyung Hyun Yoo, Xianke Zeng, Tyler Kuhns, Chul Min Yang, Teresa Mohr, Chengyu Liu, Lothar Hennighausen
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	Nature Portfolio 2017-05-01
Reeks:	Nature Communications
Online toegang:	https://doi.org/10.1038/ncomms15464
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