An advanced method for the release, enrichment and purification of high-quality Arabidopsis thaliana rosette leaf trichomes enables profound insights into the trichome proteome

Abstract Background Rosette leaf trichomes of Arabidopsis thaliana have been broadly used to study cell development, cell differentiation and, more recently, cell wall biogenesis. However, trichome-specific biochemical or -omics analyses require a proper separation of trichomes from residual plant t...

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Bibliografische Detailangaben

Hauptverfasser:	Jan W. Huebbers, Kim Büttgen, Franz Leissing, Melissa Mantz, Markus Pauly, Pitter F. Huesgen, Ralph Panstruga
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	BMC 2022-01-01
Schriftenreihe:	Plant Methods
Schlagworte:	Leaf trichome Arabidopsis thaliana Proteome Cell wall analysis Density gradient centrifugation Histochemical staining
Online-Zugang:	https://doi.org/10.1186/s13007-021-00836-0
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