An improved method for rapid generation of unmarked <it>Pseudomonas aeruginosa </it>deletion mutants
<p>Abstract</p> <p>Background</p> <p>Traditional gene replacement procedures are still time-consuming. They usually necessitate cloning of the gene to be mutated, insertional inactivation of the gene with an antibiotic resistance cassette and exchange of the plasmid-borne mutant allele with the bact...
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| Principais autores: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
BMC
2005-05-01
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| Serier: | BMC Microbiology |
| Online adgang: | http://www.biomedcentral.com/1471-2180/5/30 |
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