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Straightforward Synthesis of BHQ-3 Amine: An Azo Dark-Quencher for FRET-Based Protease Activity Assays

A Black Hole Quencher-3 (BHQ-3) derivative was synthesized through an azo-coupling reaction between Methylene Violet 3RAX and a tertiary aniline functionalized with a pendant primary amine, allowing subsequent peptide conjugation. The synthesized compounds were characterized using NMR, UV–vis absorp...

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Autores principales: Cátia D. F. Martins, Maria Manuela M. Raposo, Susana P. G. Costa
Formato: Artigo
Lenguaje:Inglês
Publicado: MDPI AG 2024-11-01
Colección:Chemistry Proceedings
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Acceso en línea:https://www.mdpi.com/2673-4583/16/1/48
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