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Rational probe design for efficient rRNA depletion and improved metatranscriptomic analysis of human microbiomes

Abstract The microbiota that colonize the human gut and other tissues are dynamic, varying both in composition and functional state between individuals and over time. Gene expression measurements can provide insights into microbiome composition and function. However, efficient and unbiased removal o...

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Principais autores: Asako Tan, Senthil Murugapiran, Alaya Mikalauskas, Jeff Koble, Drew Kennedy, Fred Hyde, Victor Ruotti, Emily Law, Jordan Jensen, Gary P. Schroth, Jean M. Macklaim, Scott Kuersten, Brice LeFrançois, Daryl M. Gohl
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2023-10-01
Serier:BMC Microbiology
Fag:
Next-generation sequencing
Microbiome
Metatranscriptomics
rRNA depletion
Online adgang:https://doi.org/10.1186/s12866-023-03037-y
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