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Cryopreservation of Rubus viruses in raspberry shoot tips via droplet-vitrification: assessment of viral preservation, localization, and post-thaw transmission capacity

Abstract We report the successful cryopreservation of three economically important Rubus viruses: raspberry bushy dwarf virus (RBDV), black raspberry necrosis virus (BRNV), and Rubus yellow net virus (RYNV), using shoot tip cryopreservation in four raspberry cultivars. Virus-infected shoot tips (app...

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Principais autores: Xiao-Yan Ma, Dag‑Ragnar Blystad, Qiao-Chun Wang, Zhibo Hamborg
Formato: Artigo
Idioma:Inglês
Publicado em: BMC 2025-10-01
coleção:Plant Methods
Assuntos:
Aphid transmission
Cryopreservation
Droplet-vitrification
In situ hybridization
Micrografting
Raspberry
Acesso em linha:https://doi.org/10.1186/s13007-025-01454-w
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