Analysis of oligonucleotide microarrays by 3′ end labeling using fluorescent nucleotides and terminal transferase
A simple enzymatic labeling procedure is described to determine spot quality in oligonucleotide microarrays. By using fluorescently labeled dideoxynucleotides or ribonucleotides as substrate for terminal deoxynucleotidyl transferase (TdT), a single fluorophore can be covalently attached at the 3′ en...
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| Autor principal: | |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Taylor & Francis Group
2006-07-01
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| Colecção: | BioTechniques |
| Acesso em linha: | https://www.future-science.com/doi/10.2144/000112182 |
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