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The promiscuous biotin ligase TurboID reveals the proxisome of the T3SS chaperone IpgC in Shigella flexneri

ABSTRACT Promiscuous biotin ligases derived from the bacterial enzyme BirA are used to identify proteins vicinal to a bait protein, thereby defining its proxisome. Despite the popularity of this approach, surprisingly little is known about its use in prokaryotes. Here, we compared the activity of fo...

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Autori principali: Nathaline Haidar-Ahmad, Kyle Tomaro, Mathieu Lavallée-Adam, François-Xavier Campbell-Valois
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2024-11-01
Serie:mSphere
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Accesso online:https://journals.asm.org/doi/10.1128/msphere.00553-24
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