A FAST AND SENSITIVE ZYMOGRAPHY METHOD OF PEROXIDASE ACTIVITY DETERMINATION USING SODIUM ACETATE BUFFER

By changing the buffer composition from phosphate to acetate (in both cases, the pH value was 6.0), the sensitivity of the estimation of peroxidase activity in polyacrylamide gels was improved from 4 µg/well to 0.4 µg/well. The optimized method, including electrophoretic separation and zymogram tec...

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Bibliografiset tiedot

Päätekijät:	Gheorghița MENGHIU, Amalia Nicoleta IANCU, Vasile OSTAFE
Aineistotyyppi:	Artigo
Kieli:	Inglês
Julkaistu:	Babes-Bolyai University 2019-06-01
Sarja:	Studia Universitatis Babes-Bolyai Chemia
Aiheet:	zymography, peroxidase, guaiacol, SDS-PAGE
Linkit:	https://studia.reviste.ubbcluj.ro/index.php/chemia/article/view/8033
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