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5-azacytidine affects triticale microspore embryogenesis and is associated with modulation of reactive oxygen species, cysteine protease profiles, and programmed cell death

Abstract Background In theory, microspore embryogenesis (ME) is the most efficient method for producing doubled haploids, however the actual effectiveness of this process is strongly genotype dependent. Here, we analysed how treatment with 5 µM 5-azacytidine (AC) is associated with early physiologic...

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Main Authors: Monika Krzewska, Agnieszka Springer, Przemysław Kopeć, Anna Nowicka, Zbyněk Milec, Kenji Yamada, Jana Moravčíková, Iwona Żur, Ewa Dubas
Format: Artigo
Language:Inglês
Published: BMC 2026-05-01
Series:BMC Plant Biology
Subjects:
5-azacytidine
Cysteine proteases
Doubled haploids
Programmed cell death
Reactive oxygen species
Microspore embryogenesis
Online Access:https://doi.org/10.1186/s12870-026-08953-5
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