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Protocol for evaluating RNA-protein associations in mammalian cells with RIP-seq and RIP-qPCR

Summary: RNA-protein interactions drive gene regulation, subcellular organization, and noncoding RNA function. Here, we present a protocol for measuring RNA-protein associations in formaldehyde-crosslinked mammalian cells using RNA immunoprecipitation followed by sequencing (RIP-seq) and quantitativ...

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Hlavní autoři: Jackson B. Trotman, Shuang Li, Quinn E. Eberhard, Zhiyue Zhang, J. Mauro Calabrese
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2026-03-01
Edice:STAR Protocols
Témata:
Bioinformatics
Sequence analysis
Sequencing
RNA-seq
ChIP-seq
Gene expression
On-line přístup:http://www.sciencedirect.com/science/article/pii/S266616672500704X
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