Molecular Attributes Associated With Refolding of Inclusion Body Proteins Using the Freeze–Thaw Method

Understanding the structure–function of inclusion bodies (IBs) in the last two decades has led to the development of several mild solubilization buffers for the improved recovery of bioactive proteins. The recently developed freeze–thaw-based inclusion body protein solubilization method has received...

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Autori principali:	Priyank Singhvi, Juhi Verma, Neha Panwar, Tabiya Qayoom Wani, Akansha Singh, Md. Qudratullah, Arnab Chakraborty, Ankit Saneja, Debi P. Sarkar, Amulya K. Panda
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Frontiers Media S.A. 2021-04-01
Serie:	Frontiers in Microbiology
Soggetti:	inclusion bodies mild solubilization method freeze–thaw method protein refolding human growth hormone protein aggregates
Accesso online:	https://www.frontiersin.org/articles/10.3389/fmicb.2021.618559/full
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