Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast

Abstract Ubiquitin‐protein ligases (E3s) are responsible for target recognition and regulate stability, localization or function of their substrates. However, the substrates of most E3 enzymes remain unknown. Here, we describe the development of a novel proteomic in vitro ubiquitination screen using...

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Detalhes bibliográficos

Principais autores:	Ronish Gupta, Bart Kus, Christopher Fladd, James Wasmuth, Raffi Tonikian, Sachdev Sidhu, Nevan J Krogan, John Parkinson, Daniela Rotin
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Springer Nature 2007-06-01
coleção:	Molecular Systems Biology
Assuntos:	endocytosis Nedd4 post‐translational modification proteomics ubiquitin ligase
Acesso em linha:	https://doi.org/10.1038/msb4100159
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