A CALB-like Cold-Active Lipolytic Enzyme from <i>Pseudonocardia antarctica</i>: Expression, Biochemical Characterization, and AlphaFold-Guided Dynamics

Cold-active lipolytic enzymes enable low-temperature biocatalysis, but remain underexplored in Antarctic actinomycetes. Here, we report the discovery and first-step characterization of a CALB-like cold-active lipolytic enzyme (PanLip) from <i>Pseudonocardia antarctica</i>. Sequence and structure ana...

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Principais autores:	Lixiao Liu, Hackwon Do, Jong-Oh Kim, Jun Hyuck Lee, Hak Jun Kim
Formato:	Artigo
Idioma:	Inglês
Publicado:	MDPI AG 2025-12-01
Series:	Marine Drugs
Assuntos:	cold-active lipolytic enzyme <i>Pseudonocardia antarctica</i> catalytic triad cold-adaptation
Acceso en liña:	https://www.mdpi.com/1660-3397/23/12/480
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