Enzymatic basis of the Fc-selective intra-chain disulfide reduction and free thiol content variability in an antibody produced in Escherichia coli

Abstract Background Escherichia coli (E. coli) is a promising host for production of recombinant proteins (including antibodies and antibody fragments) that don’t require complex post-translational modifications such as glycosylation. During manufacturing-scale production of a one-armed antibody in...

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Detalhes bibliográficos

Principais autores:	Tomasz K. Baginski, Karthik Veeravalli, Rebekah McKenna, Christopher Williams, Katherine Wong, Christina Tsai, Daniel Hewitt, Karthik Mani, Michael W. Laird
Formato:	Artigo
Idioma:	Inglês
Publicado em:	BMC 2022-08-01
coleção:	Microbial Cell Factories
Assuntos:	Antibody Disulfide bond reduction Free thiols Fc glycosylation Disulfide bond isomerase C (DsbC) Homogenate hold
Acesso em linha:	https://doi.org/10.1186/s12934-022-01892-4
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