Protein Domain Guided Screen for Sequence Specific and Phosphorothioate-Dependent Restriction Endonucleases

Modification dependent restriction endonucleases (MDREs) restrict modified DNA, typically with limited sequence specificity (∼2–4 bp). Here, we focus on MDREs that have an SRA and/or SBD (sulfur binding domain) fused to an HNH endonuclease domain, cleaving cytosine modified or phosphorothioated (PT)...

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Auteurs principaux:	Thomas Lutz, Honorata Czapinska, Alexey Fomenkov, Vladimir Potapov, Daniel F. Heiter, Bo Cao, Peter Dedon, Matthias Bochtler, Shuang-yong Xu
Format:	Artigo
Langue:	Inglês
Publié:	Frontiers Media S.A. 2020-08-01
Collection:	Frontiers in Microbiology
Sujets:	DNA backbone phosphorothioate modification PT modification dependent endonucleases SBD and HNH domain fusion EcoWI endonuclease Bsp305I endonuclease
Accès en ligne:	https://www.frontiersin.org/article/10.3389/fmicb.2020.01960/full
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