Generation of a SOX2 reporter human induced pluripotent stem cell line using CRISPR/SaCas9
SOX2 is an important transcription factor involved in pluripotency maintenance, pluripotent reprogramming and differentiation towards neural lineages. Here we engineered the previously described HEL24.3 hiPSC to generate a SOX2 reporter by knocking-in a T2A fused nuclear tdTomato reporter cassette b...
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| Huvudupphov: | , , , , , |
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| Materialtyp: | Artigo |
| Språk: | Inglês |
| Utgiven: |
Elsevier
2017-07-01
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| Serie: | Stem Cell Research |
| Länkar: | http://www.sciencedirect.com/science/article/pii/S1873506117300831 |
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