Does pre-incubation in selective-enrichment media improve the detection of diarrheagenic Escherichia coli using the RIDA®GENE PCR?

We aimed to investigate whether a selective pre-PCR enrichment step improves test performance of RIDA®GENE EHEC/EPEC to detect diarrheagenic Escherichia coli from stool samples. Each of the 250 stool samples was analyzed for the presence of stx1/2 and eae both with and without pre-PCR enrichment in...

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Bibliografische Detailangaben

Hauptverfasser:	Neele J. Froböse, Ioana D. Olaru, Julia Sophie Schneider, Wenlan Zhang, Alexander Mellmann, Franziska Schuler, Tobias Grebe, Frieder Schaumburg
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Elsevier 2023-03-01
Schriftenreihe:	International Journal of Medical Microbiology
Schlagworte:	Enteropathogenic Escherichia coli Enterohemorrhagic Escherichia coli Culture Enrichment, polymerase chain reaction
Online-Zugang:	http://www.sciencedirect.com/science/article/pii/S1438422123000036
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