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A high-efficiency PEG-Ca2+-mediated transient transformation system for broccoli protoplasts

Transient transformation of plant protoplasts is an important method for studying gene function, subcellular localization and plant morphological development. In this study, an efficient transient transformation system was established by optimizing the plasmid concentration, PEG4000 mass concentrati...

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Principais autores: Dongxu Yang, Yongyu Zhao, Yumei Liu, Fengqing Han, Zhansheng Li
格式: Artigo
語言:Inglês
出版: Frontiers Media S.A. 2022-12-01
叢編:Frontiers in Plant Science
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在線閱讀:https://www.frontiersin.org/articles/10.3389/fpls.2022.1081321/full
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