A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos.

<h4>Background</h4>The insulated isothermal PCR (iiPCR) technology enables consistent PCR amplification and detection in a simple heating device. A pan-dengue virus (DENV) RT-iiPCR, targeting the 5' untranslated region, was validated previously on the semi-automated POCKIT combo system (involving se...

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Autori principali:	Jih-Jin Tsai, Wei-Liang Liu, Ping-Chang Lin, Bo-Yi Huang, Ching-Yi Tsai, Pei-Yu Alison Lee, Yun-Long Tsai, Pin-Hsing Chou, Simon Chung, Li-Teh Liu, Chun-Hong Chen
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Public Library of Science (PLoS) 2019-01-01
Serie:	PLoS ONE
Accesso online:	https://doi.org/10.1371/journal.pone.0218139
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