Harnessing accurate non-homologous end joining for efficient precise deletion in CRISPR/Cas9-mediated genome editing

Abstract Background Many applications of CRISPR/Cas9-mediated genome editing require Cas9-induced non-homologous end joining (NHEJ), which was thought to be error prone. However, with directly ligatable ends, Cas9-induced DNA double strand breaks may be repaired preferentially by accurate NHEJ. Resu...

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Detalhes bibliográficos

Principais autores:	Tao Guo, Yi-Li Feng, Jing-Jing Xiao, Qian Liu, Xiu-Na Sun, Ji-Feng Xiang, Na Kong, Si-Cheng Liu, Guo-Qiao Chen, Yue Wang, Meng-Meng Dong, Zhen Cai, Hui Lin, Xiu-Jun Cai, An-Yong Xie
Formato:	Artigo
Idioma:	Inglês
Publicado em:	BMC 2018-10-01
Colecção:	Genome Biology
Assuntos:	Paired gRNAs CRISPR/Cas9 Accurate NHEJ Templated insertions Precise deletion Knockout
Acesso em linha:	http://link.springer.com/article/10.1186/s13059-018-1518-x
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