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Dissociation protocols used for sarcoma tissues bias the transcriptome observed in single-cell and single-nucleus RNA sequencing

Abstract Background Single-cell RNA-seq has emerged as an innovative technology used to study complex tissues and characterize cell types, states, and lineages at a single-cell level. Classification of bulk tumors by their individual cellular constituents has also created new opportunities to genera...

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Autors principals: Danh D. Truong, Salah-Eddine Lamhamedi-Cherradi, Robert W. Porter, Sandhya Krishnan, Jyothishmathi Swaminathan, Amber Gibson, Alexander J. Lazar, J. Andrew Livingston, Vidya Gopalakrishnan, Nancy Gordon, Najat C. Daw, Nicholas E. Navin, Richard Gorlick, Joseph A. Ludwig
Format: Artigo
Idioma:Inglês
Publicat: BMC 2023-05-01
Col·lecció:BMC Cancer
Matèries:
RNA-seq
scRNA-seq
snRNA-seq
Single-cell
Ewing sarcoma
Desmoplastic small round cell tumor
Accés en línia:https://doi.org/10.1186/s12885-023-10977-1
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