Metabarcoding with Illumina and Oxford Nanopore Technologies provides complementary insights into tree seed mycobiota

Abstract Background Culturing of fungi is labor-intensive and reveals limited diversity, while high-throughput sequencing of barcodes (i.e., metabarcoding) enables a simultaneous detection of fungi from multiple environmental samples. Metabarcoding using short-read sequencers, such as Illumina platf...

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Autori principali:	Jana Mittelstrass, Renate Heinzelmann, René Eschen, Martin Hartmann, Quirin Kupper, Salome Schneider, Simone Prospero, Iva Franić
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	BMC 2025-05-01
Serie:	Environmental Microbiome
Soggetti:	High-throughput sequencing Amplicon sequencing Read length MinION ITS Endophytes
Accesso online:	https://doi.org/10.1186/s40793-025-00712-7
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