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Isolation and profiling of viable tumor cells from human ex vivo glioblastoma cultures through single-cell transcriptomics

Summary: Single-cell RNA-sequencing (scRNA-seq) is becoming a ubiquitous method in profiling the cellular transcriptomes of both malignant and non-malignant cells from the human brain. Here, we present a protocol to isolate viable tumor cells from human ex vivo glioblastoma cultures for single-cell...

Whakaahuatanga katoa

I tiakina i:
Ngā kaituhi matua: Junyi Zhang, Jakob Straehle, Kevin Joseph, Nicolas Neidert, Simon Behringer, Jonathan Göldner, Andreas Vlachos, Marco Prinz, Christian Fung, Jürgen Beck, Oliver Schnell, Dieter Henrik Heiland, Vidhya M. Ravi
Hōputu: Artigo
Reo:Inglês
I whakaputaina: Elsevier 2023-09-01
Rangatū:STAR Protocols
Ngā marau:
Cell Biology
Cell Isolation
Single Cell
Flow Cytometry/Mass Cytometry
Cancer
Sequencing
Urunga tuihono:http://www.sciencedirect.com/science/article/pii/S2666166723003507
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