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Peptide tags for labeling membrane proteins in live cells with multiple fluorophores

We describe a method to label specific membrane proteins with fluorophores for live imaging. Fusion proteins are generated that incorporate into their extracellular domains short peptide sequences (13–38 amino acids) recognized with high affinity and specificity by protein ligands, α-bungarotoxin (B...

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Principais autores: Corey M. McCann, Florence M. Bareyre, Jeff W. Lichtman, Joshua R. Sanes
格式: Artigo
語言:Inglês
出版: Taylor & Francis Group 2005-06-01
叢編:BioTechniques
在線閱讀:https://www.future-science.com/doi/10.2144/05386IT02
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