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Using Live Imaging and FUCCI Embryonic Stem Cells to Rank DevTox Risks: Adverse Growth Effects of PFOA Compared With DEP Are 26 Times Faster, 1,000 Times More Sensitive, and 13 Times Greater in Magnitude

Fluorescent ubiquitination-based cell cycle indicator (FUCCI) embryonic stem cells (ESCs), which fluoresce green during the S-G2-M phases, generate an S-shaped curve for the accumulation of cells during normal stemness (NS) culture with leukemia-inhibitory factor (LIF). Since it was hypothesized tha...

Celý popis

Uloženo v:
Hlavní autoři: Mohammed Abdulhasan, Ximena Ruden, Yuan You, Sean M. Harris, Douglas M. Ruden, Awoniyi O. Awonuga, Ayesha Alvero, Elizabeth E. Puscheck, Daniel A. Rappolee
Médium: Artigo
Jazyk:Inglês
Vydáno: Frontiers Media S.A. 2021-11-01
Edice:Frontiers in Toxicology
Témata:
HTS
high through put screening
perfluorinated alkylated substances
phthalates
developmental toxicity
embryonic stem cell
On-line přístup:https://www.frontiersin.org/articles/10.3389/ftox.2021.709747/full
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