The main protease 3CLpro of the SARS-CoV-2 virus: how to turn an enemy into a helper

Despite the long history of use and the knowledge of the genetics and biochemistry of E. coli, problems are still possible in obtaining a soluble form of recombinant proteins in this system. Although, soluble protein can be obtained both in the cytoplasm and in the periplasm of the bacterial cell. T...

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Principais autores:	Svetlana V. Belenkaya, Iuliia A. Merkuleva, Olga I. Yarovaya, Varvara Yu. Chirkova, Elena A. Sharlaeva, Daniil V. Shanshin, Ekaterina A. Volosnikova, Sergey Z. Vatsadze, Mikhail V. Khvostov, Nariman F. Salakhutdinov, Dmitriy N. Shcherbakov
格式:	Artigo
語言:	Inglês
出版:	Frontiers Media S.A. 2023-06-01
叢編:	Frontiers in Bioengineering and Biotechnology
主題:	E. coli bacteria 3CL protease TEV protease RBD SARS-CoV-2
在線閱讀:	https://www.frontiersin.org/articles/10.3389/fbioe.2023.1187761/full
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