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Preparation of Pneumococcal Proteins for Western Blot Analysis

This protocol was developed in a study aimed to determine the cellular localization of the lysin of pneumococcal phage SV1 (Frias et al., 2013). We obtained proteins from the surface of Streptococcus pneumoniae by elution with choline or those secreted to the medium. The analysis by Western blot of...

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Dettagli Bibliografici
Autori principali: Maria Frias, José Melo-Cristino, Mário Ramirez
Natura: Artigo
Lingua:Inglês
Pubblicazione: Bio-protocol LLC 2013-07-01
Serie:Bio-Protocol
Accesso online:https://bio-protocol.org/en/bpdetail?id=807&type=0
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