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A Stable and Rapid Protoplast-Based CRISPR/Cas9-Ribonucleoprotein Complex Screening System for Octoploid Strawberry (<i>Fragaria × ananassa</i>)

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a simple and powerful tool, which enables gene knockout or insertion of new gene cassettes. This method has been applied to various plants and is used for crop improvement. Cultivated stra...

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Principais autores: Yunsun Kim, Jihye Jang, Youngjae Oh, Seonghee Lee, Beum-Chang Kang
Format: Artigo
Sprog:Inglês
Udgivet: MDPI AG 2026-02-01
Serier:Plants
Fag:
<i>Fragaria</i>
strawberry
protoplast
CRISPR/Cas9
gRNA screening
Online adgang:https://www.mdpi.com/2223-7747/15/4/565
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