TCRβ Clonotype Analysis of EBV and CMV-specific Human CD8+ T Cells
This protocol describes the quantification of all expressed T-cell antigen receptor (TCR) gene products within sorted (by flow cytometry) EBV and CMV-specific memory CD8+ T-cell populations using a template-switch anchored reverse transcription polymerase chain reaction (RT-PCR).
Guardat en:
| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Bio-protocol LLC
2015-10-01
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| Col·lecció: | Bio-Protocol |
| Accés en línia: | https://bio-protocol.org/en/bpdetail?id=1606&type=0 |
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