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Methods for simultaneous and quantitative isolation of mitochondrial DNA, nuclear DNA and RNA from mammalian cells

The aim of this study was to assess two protocols for their capacities to simultaneously isolate RNA, mtDNA and ncDNA from mammalian cells. We compared the Invitrogen TRIzol-based method and Qiagen DNeasy columns, using the HepG2 cell line and human primary glioblastoma stem cells. Both methods allo...

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Principais autores: Jean Nakhle, Tülin Özkan, Kateřina Lněničková, Philippe Briolotti, Marie-Luce Vignais
Format: Artigo
Sprog:Inglês
Udgivet: Taylor & Francis Group 2020-12-01
Serier:BioTechniques
Fag:
mammalian cells
mitochondria
mitochondrial DNA (mtDNA)
nuclear DNA (ncDNA)
RNA
Online adgang:https://www.future-science.com/doi/10.2144/btn-2020-0114
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