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CRISPR-PCDup: a novel approach for simultaneous segmental chromosomal duplication in Saccharomyces cerevisiae

Abstract In our previous study, a novel genome engineering technology, PCR-mediated chromosome duplication (PCDup), was developed in Saccharomyces cerevisiae that enabled the duplication of any desired chromosomal region, resulting in a segmental aneuploid. From one round of transformation, PCDup ca...

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主要な著者: Naim Hassan, Yu Sasano, Shunta Kimura, Farhana Easmin, Keisuke Ekino, Hisataka Taguchi, Satoshi Harashima
フォーマット: Artigo
言語:Inglês
出版事項: SpringerOpen 2020-02-01
シリーズ:AMB Express
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オンライン・アクセス:https://doi.org/10.1186/s13568-020-0957-4
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