Orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase
Engineering enzymes to accept noncanonical cofactor biomimetics is difficult. Here, the authors establish a self-sufficient growth selection method and demonstrate its application in engineering the Lactobacillus pentosus NADH oxidase to efficiently recycle reduced nicotinamide mononucleotide (NMNH)...
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| Principais autores: | , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Nature Portfolio
2022-11-01
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| Series: | Nature Communications |
| Acceso en liña: | https://doi.org/10.1038/s41467-022-35021-x |
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