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Rapid microfluidic isolation of virally infected primary bronchial epithelial cells for single-cell RNA sequencing

Single-cell RNA sequencing (scRNA-seq) of the bronchial epithelium enables examination of cellular subtypes and their responses to viral infections. Here, an optimized method for the isolation of virally infected primary bronchial epithelial cells using a commercially available microfluidic device i...

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主要な著者: Lucy M Kimbley, Rachel Parker, Maaike Sybil Jongen, John W Holloway, Emily J Swindle, Matthew JJ Rose-Zerilli
フォーマット: Artigo
言語:Inglês
出版事項: Taylor & Francis Group 2021-07-01
シリーズ:BioTechniques
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オンライン・アクセス:https://www.future-science.com/doi/10.2144/btn-2021-0020
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