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Improved methods for the study of hepatic HMG CoA reductase: one-step isolation of mevalonolactone and rapid preparation of endoplasmic reticulum

Two new methods are described for the study of hepatic 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase. (1) Endoplasmic reticulum was rapidly prepared by diluting a 10,000 g supernatant with buffer containing 8 mM calcium chloride. The yield of protein and the specific activity of HMG CoA...

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书目详细资料
Principais autores: C D Goodwin, S Margolis
格式: Artigo
语言:Inglês
出版: Elsevier 1976-05-01
丛编:Journal of Lipid Research
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在线阅读:http://www.sciencedirect.com/science/article/pii/S0022227520369911
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