A Workflow for High-pressure Freezing and Freeze Substitution of the Caenorhabditis elegans Embryo for Ultrastructural Analysis by Conventional and Volume Electron Microscopy
The free-living nematode Caenorhabditis elegans is a popular model system for studying developmental biology. Here we describe a detailed protocol to high-pressure freeze the C. elegans embryo (either ex vivo after dissection, or within the intact worm) followed by quick freeze substitution. Process...
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| Principais autores: | , , , |
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| 格式: | Artigo |
| 语言: | Inglês |
| 出版: |
Bio-protocol LLC
2021-04-01
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| 丛编: | Bio-Protocol |
| 在线阅读: | https://bio-protocol.org/en/bpdetail?id=3981&type=0 |
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