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A new way to rapidly create functional, fluorescent fusion proteins: random insertion of GFP with an <it>in vitro</it> transposition reaction

<p>Abstract</p> <p>Background</p> <p>The jellyfish green fluorescent protein (GFP) can be inserted into the middle of another protein to produce a functional, fluorescent fusion protein. Finding permissive sites for insertion, however, can be difficult. Here we describe a transposon-based approach f...

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I tiakina i:
Ngā taipitopito rārangi puna kōrero
Ngā kaituhi matua: Jakobsdottir Klara B, Inglis Fiona M, Robert Antoine, Berlot Catherine H, Sheridan Douglas L, Howe James R, Hughes Thomas E
Hōputu: Artigo
Reo:Inglês
I whakaputaina: BMC 2002-06-01
Rangatū:BMC Neuroscience
Urunga tuihono:http://www.biomedcentral.com/1471-2202/3/7
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