T7 RNA polymerase-guided base editor for accelerated continuous evolution in Bacillus subtilis

Targeted in vivo hypermutation mediated by base deaminase-T7 RNA polymerase (T7 RNAP) fusions promotes genetic diversification and accelerates continuous directed evolution. Due to the lack of a T7RNAP expression regulation system and functionally compatible linker for fusion protein expression, T7R...

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Detalles Bibliográficos

Principais autores:	Bin Wang, Yaokang Wu, Xueqin Lv, Long Liu, Jianghua Li, Guocheng Du, Jian Chen, Yanfeng Liu
Formato:	Artigo
Idioma:	Inglês
Publicado:	KeAi Communications Co., Ltd. 2025-09-01
Series:	Synthetic and Systems Biotechnology
Assuntos:	T7 RNA polymerase Continuous directed evolution BS-MutaT7 system Bacillus subtilis
Acceso en liña:	http://www.sciencedirect.com/science/article/pii/S2405805X25000572
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