Site-Selective Ribosylation of Fluorescent Nucleobase Analogs Using Purine-Nucleoside Phosphorylase as a Catalyst: Effects of Point Mutations

Enzymatic ribosylation of fluorescent 8-azapurine derivatives, like 8-azaguanine and 2,6-diamino-8-azapurine, with purine-nucleoside phosphorylase (PNP) as a catalyst, leads to N9, N8, and N7-ribosides. The final proportion of the products may be modulated by point mutations in the enzyme active sit...

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Bibliografiske detaljer

Principais autores:	Alicja Stachelska-Wierzchowska, Jacek Wierzchowski, Agnieszka Bzowska, Beata Wielgus-Kutrowska
Format:	Artigo
Sprog:	Inglês
Udgivet:	MDPI AG 2015-12-01
Serier:	Molecules
Fag:	fluorescent nucleosides enzymatic ribosylation 8-azapurines purine nucleoside phosphorylase
Online adgang:	http://www.mdpi.com/1420-3049/21/1/44
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