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A capture methyl-seq protocol with improved efficiency and cost-effectiveness using pre-pooling and enzymatic conversion

Abstract Objective The opportunities for sequencing-based methylome analysis of clinical samples are increasing. To reduce its cost and the amount of genomic DNA required for library preparation, we aimed to establish a capture methyl-seq protocol, which adopts pre-pooling of multiple libraries befo...

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Bibliografiset tiedot
Päätekijät: Keita Hasegawa, Kazuhiko Nakabayashi, Keisuke Ishiwata, Yoshifumi Kasuga, Kenichiro Hata, Mamoru Tanaka
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: BMC 2023-07-01
Sarja:BMC Research Notes
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Linkit:https://doi.org/10.1186/s13104-023-06401-3
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