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Stable long-term germline transmission of GFP transgenic rat via PiggyBac transposon mediated gene transfer

Abstract Transgene silencing provides a significant challenge in animal model production via gene engineering using viral vectors or transposons. Selecting an appropriate strategy, contingent upon the species is crucial to circumvent transgene silencing, necessitating long-term observation of in viv...

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Main Authors: Beom-Jin Jeon, Dong-Hyeok Kwon, Gyeong-min Gim, Hee-Kyoung Kim, Jeong-Hwa Lee, Goo Jang
Format: Artigo
Language:Inglês
Published: BMC 2024-06-01
Series:BMC Veterinary Research
Subjects:
Germline Transmission
GFP-Rats
Resources
Transgene Silencing
Transposon
Online Access:https://doi.org/10.1186/s12917-024-04123-7
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