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Polyclonal antibody-based dot-ELISA and immunocapture-RT-PCR for Cucumber green mottle mosaic virus detection

Cucumber green mottle mosaic virus (CGMMV) is one of important viruses from cucurbit crops. Using purified CGMMV particles as immunogens, the polyclonal antibody (PAb) was prepared in New Zealand rabbits. The ELISA titer of the PAb was 1∶512 000. The PAb could specifically react with the 17.5 ku coa...

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Principais autores: SHANG Hai-li, ZHOU Xue-ping, WU Jian-xiang
Format: Artigo
Sprog:Inglês
Udgivet: Zhejiang University Press 2010-09-01
Serier:浙江大学学报. 农业与生命科学版
Fag:
<italic>Cucumber green mottle mosaic virus</italic>
polyclonal antibody
dot-ELISA
IC-RT-PCR
Online adgang:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.05.002
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